awkを使用して最後の行と次の行に基づいて12を減算または合計するにはどうすればよいですか？

次のコマンドを試してください。

root@u2004:~# cat test
##sequence-region Q75T13 1 641
Q75T13,UniProtKB,Chain,1,641,.,.,.,ID
Q75T13,UniProtKB,Topological domain,1,60,.,.,.,Note=Cytoplasmic
Q75T13,UniProtKB,Transmembrane,61,85,.,.,.,Note=Helical
Q75T13,UniProtKB,Topological domain,86,641,.,.,.,Note=Lumenal


##sequence-region Q9BRR3 1 403
Q9BRR3,UniProtKB,Chain,1,403,.,.,.,ID
Q9BRR3,UniProtKB,Topological domain,1,22,.,.,.,Note=Lumenal
Q9BRR3,UniProtKB,Transmembrane,23,43,.,.,.,Note=Helical
Q9BRR3,UniProtKB,Topological domain,44,259,.,.,.,Note=Cytoplasmic

##sequence-region Q96FM1 1 250
Q96FM1,UniProtKB,Topological domain,120,135,.,.,.,Note=Cytoplasmic
Q96FM1,UniProtKB,Transmembrane,136,156,.,.,.,Note=Helical
Q96FM1,UniProtKB,Topological domain,157,169,.,.,.,Note=Lumenal
Q96FM1,UniProtKB,Transmembrane,170,190,.,.,.,Note=Helical
Q96FM1,UniProtKB,Topological domain,191,250,.,.,.,Note=Lumenal
root@u2004:~# 
root@u2004:~# awk -F, -v OFS=, '{while(1){if($0~/Lumenal/){a=$0; $4-=12;p=$0; $0=a;$5+=12;n=$0; if(index(pre,"Transmembrane")>0)print p; if(getline>0){if(index($0,"Transmembrane"))print n; if($0~/Lumenal/){pre=$0; continue}}} break}} {pre=$0}' test
Q75T13,UniProtKB,Topological domain,74,641,.,.,.,Note=Lumenal
Q9BRR3,UniProtKB,Topological domain,1,34,.,.,.,Note=Lumenal
Q96FM1,UniProtKB,Topological domain,145,169,.,.,.,Note=Lumenal
Q96FM1,UniProtKB,Topological domain,157,181,.,.,.,Note=Lumenal
Q96FM1,UniProtKB,Topological domain,179,250,.,.,.,Note=Lumenal
root@u2004:~#